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Main serum bank testing The main serum banks were tested using the recomWell Parvovirus B19 Adata RB19 assay kits Mikrogen from the same batch by five laboratories in each country according to the manufacturer's instructions.

Quantitative results were calculated from the optical densities and plate-dependent corrections according to manufacturer's instructions. The cut-off defined by the manufacturer to Adata RB19 negative from positive samples was checked and validated using histograms of the quantitative results on a logarithmic scale.

Modelling seroprevalence and FOI Seroprevalence was estimated separately for each country, sex and age group. The age groups correspond approximately to the 20th, 40th, 60th and 80th percentiles of the age distribution of serum Adata RB19.

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For the non-parametric estimates of Adata RB19 seroprevalence and FOI, local quadratic polynomials were used with a data-driven automatic procedure to choose the local bandwidth [ 9 ]. Estimating number of parvovirus B19 infections in pregnant women The age-dependent number of infections in pregnancy was estimated Adata RB19 Eurostat data of live births in http: Donors of discordant sera were significantly younger than donors of sera with concordant results.

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Antibody reactivity in the Biotrin assay on these sera was also significantly lower than for concordant positive results. Seroprofiles by age of tested sera were similar between the two assays except in the youngest age groups, where the Biotrin assay estimated a slightly higher seroprevalence. Based on these results, the Mikrogen assay was chosen for testing the five main serum banks. Analysis of the cut-off used for the main serum bank For Adata RB19 country's main serum bank, the Mikrogen assay produced two distinct populations of seropositives and seronegatives Fig.

The equivocal range indicated by the manufacturer was found in the trough between the two populations modes, such that there was no need to apply further modelling techniques as suggested in a previous study based on a different laboratory assay [ 10 ].

For further analysis all samples in the equivocal range were excluded see Table 1. It appears the controversy has arisen because the reported signatures derived from analysis of Adata RB19 tissues have not been properly validated.

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In fact, there have been no established signatures that accurately predict the survival Adata RB19 stage I lung cancer to date. Since it has been difficult to identify common key genes reflecting early stage aggressiveness by analyzing cancer tissues because of tremendous heterogeneity, we focused Adata RB19 lung epithelial cells. Epidermal growth factor EGFa major growth factor for lung epithelial cells, stimulates a variety of cellular responses with regulated expression of many genes that are known to be involved in cancer aggressiveness.


Although expression Adata RB19 of many growth factor-signaling genes are thought to be regulated in a gene network in which each gene affects other genes, there remains no way to clarify the connectivity between each gene to overview the whole network that shows dynamic changes over time. We found that most molecules Adata RB19 by the key genes are known to play important roles in cancer aggressiveness, providing a theoretical basis for prognosis prediction.

We demonstrated that the EGF-signaling key genes accurately predict the survival of patients with stage I lung cancer. The accuracy was adequately validated by using completely independent expression Adata RB19 of lung cancer derived from publically available sources. Moreover, we collected a total of stage I lung adenocarcinomas with high-quality gene-expression data and pathological and clinical information of Japanese patients, including relapse-free survival, from National Cancer Center in Japan.

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